Stat5B is required for IgE-Mediated mast cell function in vitro and in vivo.

Autor: Kiwanuka KN; Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298, United States., Motunrayo Kolawole E; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States., Mcleod JJA; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States., Baker B; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States., Paez PA; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States., Zellner MP; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Haque TT; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Paranjape A; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Jackson K; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Kee SA; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States., Dailey J; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Martin RK; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, United States., Ryan JJ; Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, United States. Electronic address: jjryan@vcu.edu.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2021 Jun; Vol. 364, pp. 104344. Date of Electronic Publication: 2021 Mar 18.
DOI: 10.1016/j.cellimm.2021.104344
Abstrakt: Mast cells are found primarily at interfaces with the external environment, where they provide protection from pathogens but also elicit allergic inflammation. Mast cell activation by antigen-induced aggregation of IgE bound to the high affinity receptor, FcεRI, is a critical factor leading to inflammation and bronchoconstriction. We previously found that Stat5 is activated by FcεRI and that Stat5B suppression decreased IgE-induced cytokine production in vitro, but in vivo responses have not been assessed. We now show that Stat5B-deficient (KO) mice have reduced responses to IgE-mediated anaphylaxis, despite normal mast cell tissue distribution. Similarly, Stat5B KO mast cells have diminished IgE-induced degranulation and cytokine secretion in vitro. These mice have elevated IgE production that is not correlated with an intrinsic B cell defect. The current work demonstrates that the Stat5B isoform is required for normal mast cell function and suggests it limits IgE production in vivo.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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