Cytokines secretion from human mesenchymal stem cells induced by bovine bone matrix.

Autor: Rodríguez-Fuentes N; CONACYT, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México., Alcántara-Quintana LE; CONACYT, Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México., Hernández-Ramírez DF; Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México., Piña-Barba MC; Instituto de Investigaciones en Materiales, UNAM, Ciudad de México, México., Cervantes-Uc JM; Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, México., Núñez-Álvarez CA; Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México.; Facultad de Medicina, UNAM, Ciudad de México, México., Ambrosio JR; Facultad de Medicina, UNAM, Ciudad de México, México.
Jazyk: angličtina
Zdroj: Bio-medical materials and engineering [Biomed Mater Eng] 2021; Vol. 32 (4), pp. 217-228.
DOI: 10.3233/BME-218000
Abstrakt: Background: Bovine bone matrix is a natural material that has been used in the treatment of bone lesions. In this study, bovine bone matrix Nukbone® (NKB) was investigated due its osteoconductive and osteoinductive properties. This biomaterial induces CBFA-1 activation and osteogenic differentiation, although the cytokines involved in these processes is still unknown.
Objective: The aim of this work was to determine the influence of NKB on the pro-osteoblastic and anti-osteoblastic cytokines secretion from human mesenchymal stem cells (hMSCs).
Methods: The hMSCs were cultured onto NKB and cytokines IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ and TNF-α were analized at 0-14 days by immunoassay. In addition, hemocompatibility of NKB and characterization of hMSCs were evaluated.
Results: NKB induces an increase on pro-osteoblastic cytokine secretion IL-4 and a decrease on anti-osteoblastic cytokine IL-6 secretion, at days 7 and 14 of cell culture. Interestingly, there was no statistical difference between secretion profiles of others cytokines analized.
Conclusions: The up-regulation of IL-4 and down-regulation of IL-6, and the secretion profiles of other cytokines examined in this work, are findings that will contribute to the understanding of the role of NKB, and similar biomaterials, in bone homeostasis and in the osteoblastic differentiation of hMSCs.
Databáze: MEDLINE
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