Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program.

Autor: Moreno BJ; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology., Aldridge RL; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology., Britch SC; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology; Seth.Britch@usda.gov., Bayer BE; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology., Kline J; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology., Hahn DA; Department of Entomology and Nematology, University of Florida., Chen C; Department of Entomology and Nematology, University of Florida., Linthicum KJ; US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology.
Jazyk: angličtina
Zdroj: Journal of visualized experiments : JoVE [J Vis Exp] 2021 Mar 12 (169). Date of Electronic Publication: 2021 Mar 12.
DOI: 10.3791/62260
Abstrakt: The control of such human diseases as dengue, Zika, and chikungunya relies on the control of their vector, the Aedes aegypti mosquito, because there is no prevention. Control of mosquito vectors can rely on chemicals applied to the immature and adult stages, which can contribute to the mortality of non-targets and more importantly, lead to insecticide resistance in the vector. The sterile insect technique (SIT) is a method of controlling populations of pests through the release of sterilized adult males that mate with wild females to produce non-viable offspring. This paper describes the process of producing sterile males for use in an operational SIT program for the control of Aedes aegypti mosquitoes. Outlined here are the steps used in the program including rearing and maintaining a colony, separating male and female pupae, irradiating and marking adult males, and shipping Aedes aegypti males to the release site. Also discussed are procedural caveats, program limitations, and future objectives.
Databáze: MEDLINE