Protocol to profile the bioenergetics of organoids using Seahorse.

Autor:	Ludikhuize MC; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands., Meerlo M; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands., Burgering BMT; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands.; Oncode Institute., Rodríguez Colman MJ; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2021 Mar 18; Vol. 2 (1), pp. 100386. Date of Electronic Publication: 2021 Mar 18 (Print Publication: 2021).
DOI:	10.1016/j.xpro.2021.100386
Abstrakt:	Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are in vitro grown self-organizing structures derived from healthy and diseased tissue that recapitulate with high fidelity the tissue of origin. Bioenergetics is commonly analyzed by Seahorse XF analysis. However, its application to organoid studies is technically challenging. Here, we share our in-house optimized protocols to examine organoid bioenergetics in response to drugs, gene knockdown, or to characterize the metabolism of specific cell types. For complete details on the use and execution of this protocol, please refer to Ludikhuize et al. (2020). Competing Interests: The authors declare no competing interests. (© 2021 The Authors.)
Databáze:	MEDLINE
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