Polymer Brush-GaAs Interface and Its Use as an Antibody-Compatible Platform for Biosensing.

Autor:	Marquez DT; Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada.; Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada., Chawich J; Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada., Hassen WM; Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada., Moumanis K; Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada., DeRosa MC; Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada., Dubowski JJ; Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2021 Mar 12; Vol. 6 (11), pp. 7286-7295. Date of Electronic Publication: 2021 Mar 12 (Print Publication: 2021).
DOI:	10.1021/acsomega.0c04954
Abstrakt:	Despite evidence showing that polymer brushes (PBs) are a powerful tool used in biosensing for minimizing nonspecific interactions, allowing for optimization of biosensing performance, and the fact that GaAs semiconductors have proven to have a remarkable potential for sensitive biomolecule detection, the combination of these two robust components has never been considered nor evaluated as a platform for biosensing applications. This work reports different methodologies to prepare and tune PBs on the GaAs interface (PB-GaAs) and their potential as useful platforms for antibody grafting, with the ultimate goal of demonstrating the innovative and attractive character of the PB-GaAs interfaces in the enhanced capture of antibodies and control of nonspecific interactions. Three different functionalization approaches were explored, one "grafting-to" and two "grafting-from," in which atom transfer radical polymerization (ATRP) was performed, followed by their corresponding characterizations. Demonstration of the compatibility of Escherichia coli ( E. coli ) and Legionella pneumophila ( Lp ) antibodies with the PB-GaAs platform compared to the results obtained with conventional biosensing architectures developed for GaAs indicates the attractive potential for operation of a sensitive biosensor. Furthermore, these results showed that by carefully choosing the nature and preparation methodology of a PB-GaAs interface, it is possible to effectively tune the affinity of PB-GaAs-based sensors toward E. coli and Lp antibodies ultimately demonstrating the superior specificity of the developed biosensing platform. Competing Interests: The authors declare no competing financial interest. (© 2021 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu