Maximizing EPS production from Pseudomonas aeruginosa and its application in Cr and Ni sequestration.

Autor:	Chug R; Amity Institute of Biotechnology, Amity University Rajasthan, NH 11C, Kant Kalwar, Jaipur, 303002, India., Mathur S; Amity Institute of Biotechnology, Amity University Rajasthan, NH 11C, Kant Kalwar, Jaipur, 303002, India., Kothari SL; Amity Institute of Biotechnology, Amity University Rajasthan, NH 11C, Kant Kalwar, Jaipur, 303002, India., Harish; Department of Botany, Mohanlal Sukhadia University Udaipur, Rajasthan, India., Gour VS; Amity Institute of Biotechnology, Amity University Rajasthan, NH 11C, Kant Kalwar, Jaipur, 303002, India.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2021 Mar 18; Vol. 26, pp. 100972. Date of Electronic Publication: 2021 Mar 18 (Print Publication: 2021).
DOI:	10.1016/j.bbrep.2021.100972
Abstrakt:	Heavy metal contamination of water bodies has been a cause of grave concern around the globe. Analysis of various industrial effluents has revealed a perilous level of Cr (VI) and Ni (II). Pseudomonas aeruginosa is an extracellular polymeric substances (EPSs) producing bacterium. EPS has a great potential in the sequestration of heavy metal ions. In the present study efforts have been made to understand the effect of time, pH, and temperature on production of EPS by P. aeruginosa (MTCC 1688). The extracted EPS has been applied for removal of Ni (II) and Cr (VI) ions from aqueous system. The results revealed that highest EPS yield (26 mg/50 mL) can be obtained after 96 h of incubation at pH 6 and 32 °C temperature in 50 mL of culture. Treatment of 10 mg/L Cr (VI) and Ni (II) with 30 mg/L EPS resulted in the removal of 26% and 9% of Cr (VI) and Ni (II), respectively. Fourier-transform infrared spectral analysis revealed the involvement of -OH, -NH, C-O, diketone, and ester functional groups of EPS in the attachment of Cr (VI) ion while involvement of amide and -C[bond, double bond]O groups in Ni (II) binding with EPS. Scaling-up the production of EPS using bioreactor may further help in developing an efficient process for treatment of water polluted with Cr and Ni. Competing Interests: The authors of this manuscript have no conflict of interest for this manuscript. (© 2021 Published by Elsevier B.V.)
Databáze:	MEDLINE
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