Murexide-derived in vitro electrochemical sensor for the simultaneous determination of neurochemicals.

Autor: Krishnan RG; Department of Chemistry, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India.; Department of Chemistry, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India., Saraswathyamma B; Department of Chemistry, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India. beenas@am.amrita.edu.; Department of Chemistry, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India. beenas@am.amrita.edu.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Nov; Vol. 413 (27), pp. 6803-6812. Date of Electronic Publication: 2021 Mar 28.
DOI: 10.1007/s00216-021-03282-y
Abstrakt: This work highlights the protocol employed for the simultaneous electroanalysis of tryptamine, serotonin and dopamine using a conducting poly-murexide-based electrode. To date, this is the first-of-its-kind report of simultaneous electrochemical determination of these three targets. Features of the developed electrode were identified by employing FE-SEM analysis. Under optimized conditions, the analytes underwent an irreversible electro-oxidation at the modified electrode surface, with a linear range of 0.5-40 μΜ, 0.4-40.4 μΜ and 0.5-40 μΜ for dopamine, serotonin and tryptamine, respectively. The electrolytic medium employed for the sensing was a phosphate-buffered solution with pH 7. The specificity of the developed electrode was also satisfactory in the presence of other biomolecules including L-phenylalanine, L-serine, glucose and ascorbic acid. Thus, the developed murexide-derived conducting-polymer-based electrode was used for the simultaneous sensing of the neurochemicals dopamine, serotonin and tryptamine. Electroanalysis was also demonstrated for these targets in human serum.
(© 2021. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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