Metabolic and oxidative impairments in human salivary gland cells line exposed to MeHg.

Autor: Nogueira LS; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil., Vasconcelos CP; Laboratory of Cell Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua, PA, Brazil., Mitre GP; School of Dentistry, Federal University of Pará, Belém, PA, Brazil., Kataoka MSDS; School of Dentistry, Federal University of Pará, Belém, PA, Brazil., Bittencourt LO; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil., Lima MO; Laboratory of Toxicology, Environment Section, Evandro Chagas Institute, Ananindeua, PA, Brazil., de Oliveira EHC; Laboratory of Cell Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua, PA, Brazil., Crespo-Lopez ME; Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil., Lima RR; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil. Electronic address: rafalima@ufpa.br.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2021 Jul; Vol. 66, pp. 126747. Date of Electronic Publication: 2021 Mar 18.
DOI: 10.1016/j.jtemb.2021.126747
Abstrakt: Background/aim: The ingestion of contaminated seafood by MeHg is considered the main route of human exposure, turning the salivary gland one important target organ. The salivary glands play critical roles in maintaining oral health homeostasis, producing saliva that maintains the oral microbiota, initiation of the digestion of macromolecules, and being essential in maintaining the integrity of the adjacent soft tissues and teeth. Thus, this study aimed to investigate the effects of MeHg exposure on human salivary gland cells line.
Methods: Cells were exposed to 1-6 μM of MeHg for 24 h, and analysis of toxicity was performed. Based on these results, the LC50 was calculated and two concentrations were chosen (0.25 and 2.5 μM MeHg) to evaluate intracellular mercury (Hg) accumulation (THg), metabolic viability and oxidative stress parameters (GSH:GSSG ratio, lipid peroxidation, protein oxidation and DNA damage).
Results: The results demonstrated accumulation of THg as we increased the MeHg concentrations in the exposure and, the higher the dose, the lower is the cell metabolic response. In addition, the 2.5 μM MeHg concentration also triggered oxidative stress in human salivary gland cells by depleting the antioxidant competence of GSH:GSSG ratio and increasing lipid peroxidation and proteins carbonyl levels, but no damages to DNA integrity.
Conclusion: In conclusion, although these two elected doses did not show lethal effects, the highest dose triggered oxidative stress and new questionings about long-term exposure models are raised to investigate furthers cellular damages to human salivary gland cells caused by MeHg exposure to extrapolate in a translational perspective.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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