The role of Escherichia coli FhlA transcriptional activator in generation of proton motive force and F O F 1 -ATPase activity at pH 7.5.

Autor: Gevorgyan H; Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, Yerevan, Armenia.; Faculty of Biology, Scientific-Research Institute of Biology, Yerevan State University, Yerevan, Armenia.; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, Yerevan, Armenia., Khalatyan S; Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, Yerevan, Armenia.; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, Yerevan, Armenia.; Laboratory of Neuroscience, Yerevan State Medical University, Yerevan, Armenia., Vassilian A; Department of Ecology and Nature Protection, Faculty of Biology, Yerevan State University, Yerevan, Armenia., Trchounian K; Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, Yerevan, Armenia.; Faculty of Biology, Scientific-Research Institute of Biology, Yerevan State University, Yerevan, Armenia.; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, Yerevan, Armenia.
Jazyk: angličtina
Zdroj: IUBMB life [IUBMB Life] 2021 Jun; Vol. 73 (6), pp. 883-892. Date of Electronic Publication: 2021 Apr 07.
DOI: 10.1002/iub.2470
Abstrakt: Escherichia coli is able to utilize the mixture of carbon sources and produce molecular hydrogen (H 2 ) via formate hydrogen lyase (FHL) complexes. In current work role of transcriptional activator of formate regulon FhlA in generation of fermentation end products and proton motive force, N'N'-dicyclohexylcarbodiimide (DCCD)-sensitive ATPase activity at 20 and 72 hr growth during utilization of mixture of glucose, glycerol, and formate were investigated. It was shown that in fhlA mutant specific growth rate was ~1.5 fold lower compared to wt, while addition of DCCD abolished the growth in fhlA but not in wt. Formate was not utilized in fhlA mutant but wt cells simultaneously utilized formate with glucose. Glycerol utilization started earlier (from 2 hr) in fhlA than in wt. The DCCD-sensitive ATPase activity in wt cells membrane vesicles increased ~2 fold at 72 hr and was decreased 70% in fhlA. Addition of formate in the assays increased proton ATPase activity in wt and mutant strain. FhlA absence mainly affected the ΔpH but not ΔΨ component of Δp in the cells grown at 72 hr but not in 24 hr. The Δp in wt cells decreased from 24 to 72 hr of growth ~40 mV while in fhlA mutant it was stable. Taken together, it is suggested that FhlA regulates the concentration of fermentation end products and via influencing F O F 1 -ATPase activity contributes to the proton motive force generation.
(© 2021 International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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