A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane.

Autor:	Mageswaran SK; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.; Department of Biophysics and Biochemistry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Yang WY; Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan. jensen@caltech.edu weiyang@gate.sinica.edu.tw., Chakrabarty Y; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Oikonomou CM; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Jensen GJ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. jensen@caltech.edu weiyang@gate.sinica.edu.tw.; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84604, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2021 Mar 26; Vol. 7 (13). Date of Electronic Publication: 2021 Mar 26 (Print Publication: 2021).
DOI:	10.1126/sciadv.abc6345
Abstrakt:	Cryo-electron tomography (cryo-ET) provides structural context to molecular mechanisms underlying biological processes. Although straightforward to implement for studying stable macromolecular complexes, using it to locate short-lived structures and events can be impractical. A combination of live-cell microscopy, correlative light and electron microscopy, and cryo-ET will alleviate this issue. We developed a workflow combining the three to study the ubiquitous and dynamic process of shedding in response to plasma membrane damage in HeLa cells. We found filopodia-like protrusions enriched at damage sites and acting as scaffolds for shedding, which involves F-actin dynamics, myosin-1a, and vacuolar protein sorting 4B (a component of the 'endosomal sorting complex required for transport' machinery). Overall, shedding is more complex than current models of vesiculation from flat membranes. Its similarities to constitutive shedding in enterocytes argue for a conserved mechanism. Our workflow can also be adapted to study other damage response pathways and dynamic cellular events. (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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