Molecular detection of Giardia duodenalis and Cryptosporidium spp. from stray dogs residing in monasteries in Bangkok, Thailand.

Autor: Khine NO; Bio-Veterinary Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand., Chimnoi W; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand., Kamyingkird K; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand., Kengradomkij C; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand., Saetiew N; Department of Animal Science, Faculty of Science and Technology, Muban Chombueng Rajabhat University, Ratchaburi 70120, Thailand., Simking P; Division of Animal Health Science, Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand., Saengow S; Division of Animal Health Science, Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand., Jittapalapong S; Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand., Inpankaew T; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand. Electronic address: tawin.i@ku.th.
Jazyk: angličtina
Zdroj: Parasitology international [Parasitol Int] 2021 Aug; Vol. 83, pp. 102337. Date of Electronic Publication: 2021 Mar 23.
DOI: 10.1016/j.parint.2021.102337
Abstrakt: Both Cryptosporidium spp. and Giardia duodenalis are enteric protozoan parasites that infect a wide variety of domestic animals as well as humans worldwide, causing diarrheal diseases. Giardia duodenalis assemblages C and D are specific to canine hosts and zoonotic assemblages A and B are also found in dogs as a reservoir host. In dogs, Cryptosporidium canis is the host-specific species while humans are infected by C. hominis and C. parvum and at least another 16 zoonotic Cryptosporidium species have been reported causing human infections, with C. meleagridis, C. viatorum, and C. ubiquitum being the most frequent. The objective of this study was to determine the prevalence of Cryptosporidium spp. and G. duodenalis from stray dogs in areas of Bangkok and to identify the species and assemblages. Fecal samples (540) were collected from dogs residing in 95 monasteries in 48 districts in the Bangkok metropolitan area. Nested Polymerase Chain Reaction (PCR) was performed using the ssu-rRNA gene for both parasites. In total, 3.0% (16/540) samples were positive for G. duodenalis, with most being G. duodenalis assemblage D (7/16) followed by assemblage C (7/16) and zoonotic assemblage A (2/16). The prevalence of Cryptosporidium spp. was 0.7% (4/540) based on the PCR results and all were the dog genotype C. canis. These results indicated that dogs residing in Bangkok monasteries poses a limited role as source of human giardiosis and cryptosporidiosis.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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