Priming of mesenchymal stem cells with a hydrosoluble form of curcumin allows keeping their mesenchymal properties for cell-based therapy development.

Autor: Colin M; Department of Pharmacotherapy and Pharmaceuticals, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Brussels, Belgium.; RD3- Pharmacognosy, Bioanalysis and Drug Discovery Unit and Analytical Platform, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Brussels, Belgium., Dechêne L; Department of Clinical Sciences, Anaesthesiology and Equine Surgery, Faculty of Veterinary Medicine, B41, University of Liege, Sart Tilman, Liège, Belgium.; Unité de Recherche en Biologie Cellulaire (URBC) - Namur Research Institute for Life Sciences (Narilis), University of Namur (UNamur), Namur, Belgium., Ceusters J; Centre of Oxygen, Research and Development, Institute of Chemistry B6a, University of Liege (ULiège), Sart Tilman, Liège, Belgium., Niesten A; Centre of Oxygen, Research and Development, Institute of Chemistry B6a, University of Liege (ULiège), Sart Tilman, Liège, Belgium., Demazy C; Unité de Recherche en Biologie Cellulaire (URBC) - Namur Research Institute for Life Sciences (Narilis), University of Namur (UNamur), Namur, Belgium., Lagneaux L; Laboratory of Clinical Cell Therapy, ULB-Research Cancer Center (U-CRC), Jules Bordet Institute, Université libre de Bruxelles (ULB), Brussels, Belgium., Zouaoui Boudjeltia K; Laboratory of Experimental Medicine, Université libre de Bruxelles, CHU de Charleroi, Hôpital Vésale, Montigny-le-Tilleul, Belgium., Franck T; Centre of Oxygen, Research and Development, Institute of Chemistry B6a, University of Liege (ULiège), Sart Tilman, Liège, Belgium., Van Antwerpen P; RD3- Pharmacognosy, Bioanalysis and Drug Discovery Unit and Analytical Platform, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Brussels, Belgium., Renard P; Unité de Recherche en Biologie Cellulaire (URBC) - Namur Research Institute for Life Sciences (Narilis), University of Namur (UNamur), Namur, Belgium., Mathieu V; Department of Pharmacotherapy and Pharmaceuticals, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Brussels, Belgium., Serteyn D; Department of Clinical Sciences, Anaesthesiology and Equine Surgery, Faculty of Veterinary Medicine, B41, University of Liege, Sart Tilman, Liège, Belgium.; Centre of Oxygen, Research and Development, Institute of Chemistry B6a, University of Liege (ULiège), Sart Tilman, Liège, Belgium.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2021 May; Vol. 25 (10), pp. 4877-4881. Date of Electronic Publication: 2021 Mar 26.
DOI: 10.1111/jcmm.16403
Abstrakt: Mesenchymal stem cells are increasingly studied for their use as drug-carrier in addition to their intrinsic potential for regenerative medicine. They could be used to transport molecules with a poor bioavailability such as curcumin in order to improve their clinical usage. This natural polyphenol, well-known for its antioxidant and anti-inflammatory properties, has a poor solubility that limits its clinical potential. For this purpose, the use of NDS27, a curcumin salt complexed with hydroxypropyl-beta-cyclodextrin (HPβCD), displaying an increased solubility in aqueous solution, is preferred. This study aims to evaluate the uptake of NDS27 into skeletal muscle-derived mesenchymal stem cells (mdMSCs) and the effects of such uptake onto their mesenchymal properties. It appeared that the uptake of NDS27 into mdMSCs is concentration-dependent and not time-dependent. The use of a concentration of 7 µmol/L which does not affect the viability and proliferation also allows preservation of their adhesion, invasion and T cell immunomodulatory abilities.
(© 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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