Candidate Rlm6 resistance genes against Leptosphaeria. maculans identified through a genome-wide association study in Brassica juncea (L.) Czern.

Autor: Yang H; School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Mohd Saad NS; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Ibrahim MI; Department of Genetics, University of Okara, Okara, Pakistan., Bayer PE; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Neik TX; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Severn-Ellis AA; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Pradhan A; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Tirnaz S; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Edwards D; School of Biological Sciences, University of Western Australia, Perth, WA, Australia., Batley J; School of Biological Sciences, University of Western Australia, Perth, WA, Australia. jacqueline.batley@uwa.edu.au.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2021 Jul; Vol. 134 (7), pp. 2035-2050. Date of Electronic Publication: 2021 Mar 25.
DOI: 10.1007/s00122-021-03803-4
Abstrakt: Key Message: One hundred and sixty-seven B. juncea varieties were genotyped on the 90K Brassica assay (42,914 SNPs), which led to the identification of sixteen candidate genes for Rlm6. Brassica species are at high risk of severe crop loss due to pathogens, especially Leptosphaeria maculans (the causal agent of blackleg). Brassica juncea (L.) Czern is an important germplasm resource for canola improvement, due to its good agronomic traits, such as heat and drought tolerance and high blackleg resistance. The present study is the first using genome-wide association studies to identify candidate genes for blackleg resistance in B. juncea based on genome-wide SNPs obtained from the Illumina Infinium 90 K Brassica SNP array. The verification of Rlm6 in B. juncea was performed through a cotyledon infection test. Genotyping 42,914 single nucleotide polymorphisms (SNPs) in a panel of 167 B. juncea lines revealed a total of seven SNPs significantly associated with Rlm6 on chromosomes A07 and B04 in B. juncea. Furthermore, 16 candidate Rlm6 genes were found in these regions, defined as nucleotide binding site leucine-rich-repeat (NLR), leucine-rich repeat RLK (LRR-RLK) and LRR-RLP genes. This study will give insights into the blackleg resistance in B. juncea and facilitate identification of functional blackleg resistance genes which can be used in Brassica breeding.
Databáze: MEDLINE