An early-morning gene network controlled by phytochromes and cryptochromes regulates photomorphogenesis pathways in Arabidopsis.

Autor: Balcerowicz M; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Mahjoub M; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Nguyen D; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Lan H; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Stoeckle D; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Conde S; Department of Statistics, University of Warwick, Coventry, UK; Alan Turing Institute, London, UK., Jaeger KE; Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, Germany., Wigge PA; Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, Germany; Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany., Ezer D; Alan Turing Institute, London, UK; Department of Biology, University of York, York YO10 5DD, UK. Electronic address: daphne.ezer@york.ac.uk.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2021 Jun 07; Vol. 14 (6), pp. 983-996. Date of Electronic Publication: 2021 Mar 23.
DOI: 10.1016/j.molp.2021.03.019
Abstrakt: Light perception at dawn plays a key role in coordinating multiple molecular processes and in entraining the plant circadian clock. The Arabidopsis mutant lacking the main photoreceptors, however, still shows clock entrainment, indicating that the integration of light into the morning transcriptome is not well understood. In this study, we performed a high-resolution RNA-sequencing time-series experiment, sampling every 2 min beginning at dawn. In parallel experiments, we perturbed temperature, the circadian clock, photoreceptor signaling, and chloroplast-derived light signaling. We used these data to infer a gene network that describes the gene expression dynamics after light stimulus in the morning, and then validated key edges. By sampling time points at high density, we are able to identify three light- and temperature-sensitive bursts of transcription factor activity, one of which lasts for only about 8 min. Phytochrome and cryptochrome mutants cause a delay in the transcriptional bursts at dawn, and completely remove a burst of expression in key photomorphogenesis genes (HY5 and BBX family). Our complete network is available online (http://www-users.york.ac.uk/∼de656/dawnBurst/dawnBurst.html). Taken together, our results show that phytochrome and cryptochrome signaling is required for fine-tuning the dawn transcriptional response to light, but separate pathways can robustly activate much of the program in their absence.
(Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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