Correlation analysis between virtual and Complement-Dependent-Cytotoxicity crossmatch in a monocenter retrospective series of 118 allografted patients.

Autor: Crocchiolo R; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy. Electronic address: roberto.crocchiolo@ospedaleniguarda.it., Lo Po' S; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Lumia D; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Lando G; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Cornacchini G; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Crucitti L; Hematology, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Pugliano MT; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Volpato E; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Cuppari I; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Sommaruga E; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Pipitone MG; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Labate S; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Grillo G; Bone Marrow Transplantation Unit, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Zucchetti E; Bone Marrow Transplantation Unit, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy., Rossini S; SIMT, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy.
Jazyk: angličtina
Zdroj: Current research in translational medicine [Curr Res Transl Med] 2021 May; Vol. 69 (2), pp. 103287. Date of Electronic Publication: 2021 Mar 23.
DOI: 10.1016/j.retram.2021.103287
Abstrakt: Purpose of the Study: The detection of patients' anti-HLA antibodies before allogeneic hematopoietic stem cell transplantation (HSCT) may affect post-transplant outcome, due to a potential detrimental impact on engraftment or toxicity-related issues. Crossmatch (XM) techniques provide support to physicians during the pre-transplant phase but the role of Complement-Dependent Cytotoxicity XM (CDC-XM) is not well-defined when performed routinely and in parallel with the virtual XM.
Patients and Methods: We report here our experience with both virtual and CDC-XM tests on n = 118 patients undergoing search for a donor other than HLA-identical sibling from July 2013 to June 2018 at our Institution. When anti-HLA antibodies (Abs) were present, they were classified as donor-specific Abs (DSA) or non-DSA.
Results: On the n = 118 patients, n = 35 (29.7 %) had a positive virtual XM test (of which one of more DSA were found in n = 8; 6.8 %) and n = 5 had a positive CDC-XM test. These latter, positive for HLA class II only, were interpreted as false-positive results due to prior administration of anti-CD20 to the patients, all affected by lymphoma; none of them had a positive virtual XM for anti-HLA Abs of class II. Importantly, all these patients successfully engrafted, further supporting the lack of significant impact of CDC-XM positive results in this series.
Conclusions: According to our data on more than a hundred patients, routinely performed CDC-XM does not seem to add significant information with respect to virtual XM. We cannot exclude the usefulness of CDC-XM in specific situations, although a positive CDC-XM result was an unfrequent event.
(Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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