Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies.

Autor: Lazarević M; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia., Djedovic N; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia., Stanisavljević S; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia., Dimitrijević M; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia., Stegnjaić G; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia., Krishnamoorthy G; Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany., Mostarica Stojković M; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotića 1, 11000 Belgrade, Serbia., Miljković Đ; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: djordjem@ibiss.bg.ac.rs., Jevtić B; Department of Immunology, Institute for Biological Research 'Siniša Stanković' - National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
Jazyk: angličtina
Zdroj: Journal of neuroimmunology [J Neuroimmunol] 2021 May 15; Vol. 354, pp. 577547. Date of Electronic Publication: 2021 Mar 16.
DOI: 10.1016/j.jneuroim.2021.577547
Abstrakt: Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8 + CD11bc + MHC class II + cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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