| Autor: |
Campion EM; Department of Life Science, Faculty of Science, Institute of Technology Sligo, Ash Lane, Co. Sligo, Ireland. campion.eva@itsligo.ie., Loughran ST; Department of Applied Sciences, Dundalk Institute of Technology, Dundalk, Co. Louth, Ireland. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2283, pp. 61-74. |
| DOI: |
10.1007/978-1-0716-1302-3_8 |
| Abstrakt: |
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a rapid detection technology that allows the amplification and quantification of specific RNA transcripts. RT-qPCR has increasingly been adopted for the detection and quantification of H. pylori across a range of sample types and applications. In addition, it is widely used to monitor host gene expression in cells and tissues in response to H. pylori infection . Outlined here is a two-step protocol that can be employed to analyze gene expression in H. pylori or H. pylori-infected samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
