| Autor: |
Malabad JCM; College of Medicine, University of the Philippines, Manila., Ang CF; College of Medicine, University of the Philippines, Manila., Palabrica FAR; College of Medicine, University of the Philippines, Manila., Cajucom MAM; College of Medicine, University of the Philippines, Manila., Gloriani NG; College of Public Health, University of the Philippines, Manila, The Philippines., Villanueva SYAM; College of Public Health, University of the Philippines, Manila, The Philippines., Montoya JC; College of Medicine, University of the Philippines, Manila. |
| Jazyk: |
angličtina |
| Zdroj: |
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2021 Apr 01; Vol. 25 (4), pp. 285-291. |
| DOI: |
10.5588/ijtld.20.0878 |
| Abstrakt: |
BACKGROUND: TB is the leading cause of death from a single infectious disease, particularly among people living with HIV (PLHIV). Molecular epidemiology provides information on prevalent genotypes of Mycobacterium tuberculosis and disease transmission dynamics, which aid in TB control. Identification of mutations that confer drug resistance is essential for the rapid diagnosis of drug-resistant TB, especially in high TB burden settings, like the Philippines. METHODS: This study aimed to determine mutations in M. tuberculosis drug resistance-conferring genes and circulating genotypes in PLHIV. MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem repeats) typing using a set of 24-loci and sequencing of drug resistance-conferring genes were performed in 22 M. tuberculosis isolates from TB-HIV co-infected patients. RESULTS: The prevalence of resistance to any drug was 31.8%, 18.2% for isoniazid monoresistance, 4.5% for streptomycin monoresistance and 9.1% for multidrug resistance. The identified mutations in the kat G , rpo B , pnc A , rps L and gyr A genes have been reported in the literature; none was found in the inh A and emb B genes. All isolates belonged to the EAI2-Manila family and were grouped into four clusters based on their phenotypic drug resistance and mutation profiles. CONCLUSION: The use of 24-loci set may be used as a more discriminatory MIRU-VNTR typing in settings where the East African-Indian lineage is predominant, like the Philippines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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