| Autor: |
Lanka P; Department of Physics, Politecnico di Milano, Milan, Italy., Francis KJ; Multi-Modality Medical Imaging Group, University of Twente, Enschede, The Netherlands.; Biomedical Photonic Imaging Group Technical Medical Centre, University of Twente, Enschede, The Netherlands., Kruit H; Multi-Modality Medical Imaging Group, University of Twente, Enschede, The Netherlands., Farina A; Institute of Photonics and Nanotechnologies, National Research Council, Milan, Italy. andrea.farina@cnr.it., Cubeddu R; Department of Physics, Politecnico di Milano, Milan, Italy., Sekar SKV; Biophotonics@Tyndall, IPIC, Tyndall National Institute, Lee Maltings, Dyke Parade, Cork, Ireland., Manohar S; Multi-Modality Medical Imaging Group, University of Twente, Enschede, The Netherlands., Pifferi A; Department of Physics, Politecnico di Milano, Milan, Italy.; Institute of Photonics and Nanotechnologies, National Research Council, Milan, Italy. |
| Abstrakt: |
Accurate monitoring of treatment is crucial in minimally-invasive radiofrequency ablation in oncology and cardiovascular disease. We investigated alterations in optical properties of ex-vivo bovine tissues of the liver, heart, muscle, and brain, undergoing the treatment. Time-domain diffuse optical spectroscopy was used, which enabled us to disentangle and quantify absorption and reduced scattering spectra. In addition to the well-known global (1) decrease in absorption, and (2) increase in reduced scattering, we uncovered new features based on sensitive detection of spectral changes. These absorption spectrum features are: (3) emergence of a peak around 840 nm, (4) redshift of the 760 nm deoxyhemoglobin peak, and (5) blueshift of the 970 nm water peak. Treatment temperatures above 100 °C led to (6) increased absorption at shorter wavelengths, and (7) further decrease in reduced scattering. This optical behavior provides new insights into tissue response to thermal treatment and sets the stage for optical monitoring of radiofrequency ablation. |
