The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.

Autor: Olafson PU; Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA. pia.olafson@usda.gov., Aksoy S; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA., Attardo GM; Department of Entomology and Nematology, University of California - Davis, Davis, CA, USA., Buckmeier G; Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA., Chen X; The Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Coates CJ; Department of Entomology, Texas A & M University, College Station, TX, USA., Davis M; Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA., Dykema J; Department of Biological Sciences, Wayne State University, Detroit, MI, USA., Emrich SJ; Department of Electrical Engineering & Computer Science, University of Tennessee, Knoxville, TN, USA., Friedrich M; Department of Biological Sciences, Wayne State University, Detroit, MI, USA., Holmes CJ; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA., Ioannidis P; Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, 1211, Geneva, Switzerland., Jansen EN; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA., Jennings EC; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA., Lawson D; The European Molecular Biology Laboratory, The European Bioinformatics Institute, The Wellcome Genome Campus, Hinxton, CB10 1SD, UK., Martinson EO; Department of Entomology, University of Georgia, Athens, GA, USA., Maslen GL; The European Molecular Biology Laboratory, The European Bioinformatics Institute, The Wellcome Genome Campus, Hinxton, CB10 1SD, UK., Meisel RP; Department of Biology and Biochemistry, University of Houston, Houston, TX, USA., Murphy TD; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Nayduch D; Arthropod-borne Animal Diseases Research Unit, USDA-ARS, Manhattan, KS, USA., Nelson DR; Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA., Oyen KJ; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA., Raszick TJ; Department of Entomology, Texas A & M University, College Station, TX, USA., Ribeiro JMC; Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Rockville, MD, USA., Robertson HM; Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL, USA., Rosendale AJ; Department of Biology, Mount St. Joseph University, Cincinnati, OH, USA., Sackton TB; Informatics Group, Faculty of Arts and Sciences, Harvard University, Cambridge, MA, USA., Saelao P; Livestock Arthropod Pests Research Unit, USDA-ARS, Kerrville, TX, USA., Swiger SL; Department of Entomology, Texas A&M AgriLife Research and Extension Center, Stephenville, TX, USA., Sze SH; Department of Computer Science & Engineering, Department of Biochemistry & Biophysics, Texas A & M University, College Station, TX, USA., Tarone AM; Department of Entomology, Texas A & M University, College Station, TX, USA., Taylor DB; Agroecosystem Management Research Unit, USDA-ARS, Lincoln, NE, USA., Warren WC; University of Missouri, Bond Life Sciences Center, Columbia, MO, USA., Waterhouse RM; Department of Ecology and Evolution, University of Lausanne, and Swiss Institute of Bioinformatics, 1015, Lausanne, Switzerland., Weirauch MT; Center for Autoimmune Genomics and Etiology, Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Werren JH; Department of Biology, University of Rochester, Rochester, NY, USA., Wilson RK; Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.; College of Medicine, Ohio State University, Columbus, OH, USA., Zdobnov EM; Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, 1211, Geneva, Switzerland., Benoit JB; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA. joshua.benoit@uc.edu.
Jazyk: angličtina
Zdroj: BMC biology [BMC Biol] 2021 Mar 10; Vol. 19 (1), pp. 41. Date of Electronic Publication: 2021 Mar 10.
DOI: 10.1186/s12915-021-00975-9
Abstrakt: Background: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies.
Results: This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways.
Conclusions: The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha.
Databáze: MEDLINE
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