Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions.

Autor:	Everetts NJ; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.; Department of Electrical Engineering & Computer Science, Center for Computational Biology, UC Berkeley, University of California, Berkeley, Berkeley, United States., Worley MI; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States., Yasutomi R; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States., Yosef N; Department of Electrical Engineering & Computer Science, Center for Computational Biology, UC Berkeley, University of California, Berkeley, Berkeley, United States., Hariharan IK; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Mar 22; Vol. 10. Date of Electronic Publication: 2021 Mar 22.
DOI:	10.7554/eLife.61276
Abstrakt:	In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, we visualized gene expression using a multilayered model of the wing disc and cataloged ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the fibroblast growth factor ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, defined by AMP-specific targets Neurotactin and midline , that is critical for proper formation of direct flight muscles. More generally, our annotated temporal cell atlas provides an organ-wide view of potential cell-cell interactions between epithelial and myogenic cells. Competing Interests: NE, MW, RY, NY, IH No competing interests declared (© 2021, Everetts et al.)
Databáze:	MEDLINE
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