Co-fractionation/mass spectrometry to identify protein complexes.
| Autor: | McWhite CD; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Papoulas O; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Drew K; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Dang V; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Leggere JC; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Sae-Lee W; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA., Marcotte EM; Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Mar 10; Vol. 2 (1), pp. 100370. Date of Electronic Publication: 2021 Mar 10 (Print Publication: 2021). |
| DOI: | 10.1016/j.xpro.2021.100370 |
| Abstrakt: | Co-fractionation/mass spectrometry (CF/MS) is a flexible and powerful method to detect physical associations of proteins. CF/MS can be applied to any tissue or organism without the need for protein-specific antibodies or epitope tags. Here, we outline two alternate protocols for MS preparation of samples (containing low or high salt) and a computational pipeline (cfmsflow) that together allow the successful application of this approach. These protocols are based on CF/MS of over 16 diverse organisms including plants and animals. For complete details on the use and execution of this protocol, please refer to McWhite et al. (2020). Competing Interests: The authors have no competing interests. (© 2021 The Authors.) |
| Databáze: | MEDLINE |
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