Usefulness of the Ranking Technique in the Microscopic Agglutination Test (MAT) to Predict the Most Likely Infecting Serogroup of Leptospira .

Autor:	Guedes IB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., de Souza GO; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Castro JFP; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Cavalini MB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., de Souza Filho AF; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Heinemann MB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2021 Mar 03; Vol. 8, pp. 654034. Date of Electronic Publication: 2021 Mar 03 (Print Publication: 2021).
DOI:	10.3389/fvets.2021.654034
Abstrakt:	The microscopic agglutination test (MAT) used for the serological diagnosis of leptospirosis, as a robust and inexpensive method, is still the reality in many laboratories worldwide. Both the performance and the interpretation of the MAT vary from region to region, making standardization difficult. The prediction of the probable infecting serogroup using this test is indispensable for elucidating the epidemiology of the disease; however, in veterinary medicine, many studies consider any reaction detected with a titer of 100, which may ultimately overestimate some serogroups. Thus, the aim of this study was to evaluate the usefulness of the ranking technique for predicting the probable infecting serogroup identified by the MAT, eliminating cross reactions with other serogroups. Leptospira strains (12 samples) were inoculated in hamsters, and after 30 days, serology was performed by the MAT for these animals to confirm the infecting serogroup. Using the ranking technique, the probable infectious serogroup found with the MAT was the same as that in which the strains of inoculated leptospires belonged; additionally, the technique can be applied in epidemiological studies involving herds. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Guedes, Souza, Castro, Cavalini, de Souza Filho and Heinemann.)
Databáze:	MEDLINE
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