Are Silver Nanoparticles Useful for Treating Second-Degree Burns? An Experimental Study in Rats.

Autor: Moreno DAN; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Saladini MS; Veterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Viroel FJM; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Dini MMJ; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Pickler TB; Laboratory for Toxicological Research (Lapetox), University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Amaral Filho J; Veterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Dos Santos CA; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Hanai-Yoshida VM; Graduate Program in Environmental and Technological Processes, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Grotto D; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.; Graduate Program in Environmental and Technological Processes, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Gerenutti M; School of Medical Sciences of the Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, SP, Brazil., Hyslop S; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil., Oshima-Franco Y; Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
Jazyk: angličtina
Zdroj: Advanced pharmaceutical bulletin [Adv Pharm Bull] 2021 Jan; Vol. 11 (1), pp. 130-136. Date of Electronic Publication: 2020 Nov 07.
DOI: 10.34172/apb.2021.014
Abstrakt: Purpose: In this work, the potential usefulness of silver nanoparticles (AgNPs) for treating burn wounds was examined. Methods: Second-degree burns were induced in male Wistar rats by touching the skin with a heated (70°C) metallic device for 10 s, after which the animals were randomly allocated to one of two groups: control (n=8, treated with sterile saline) and experimental (n=8, treated with AgNPs, 0.081 mg/mL; 50 µL applied to the burn surface). Seven, 14, 21 and 28 days after lesion induction two rats from each group were killed and blood samples were collected for a complete blood count and to assess oxidative stress. The livers were examined macroscopically and skin samples were collected for histological analysis. Results: Macroscopically, wound healing and skin remodeling in the experimental group were similar to the saline-treated rats. Likewise, there were no significant differences in the histological parameters between the two groups. However, treatment with AgNPs caused a persistent reduction in white blood cell (WBC) counts throughout the experiment, whereas platelet counts increased on days 7 and 28 but decreased on days 14 and 21; there was also an increase in the blood concentration of reduced glutathione on day 7 followed by a decrease on days 21 and 28. There were no significant changes in blood glutathione peroxidase (GSH-Px) and catalase (CAT) activities or in the serum concentration of thiobarbituric acid reactive substances. Conclusion: The findings of this study raise questions about the potential transitory effects of AgNPs based on the changes in WBC and platelet counts, blood glutathione concentrations and macroscopic hepatic alterations.
(© 2021 The Authors.)
Databáze: MEDLINE
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