Anti-inflammatory Effects of S. cumini Seed Extract on Gelatinase-B (MMP-9) Regulation against Hyperglycemic Cardiomyocyte Stress.

Autor: Atale N; Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307 Uttar Pradesh, India., Mishra CB; College of Pharmacy, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-13 Gu, Seoul 140-742, Republic of Korea., Kohli S; Institute for Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig (Medical Faculty), Leibigstr. 27, 04103 Leipzig, Germany., Mongre RK; College of Pharmacy, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-13 Gu, Seoul 140-742, Republic of Korea., Prakash A; Amity Institute of Integrative Sciences and Health, Amity University, Gurgaon 122413, Haryana, India., Kumari S; Division of Biochemistry, Indian Agricultural Research Institute (IARI), Pusa Campus, 110012, New Delhi, India., Yadav UCS; School of Life Sciences, Central University of Gujarat, Sector-30, Gandhinagar, 382030 Gujarat, India., Jeon R; College of Pharmacy, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-13 Gu, Seoul 140-742, Republic of Korea., Rani V; Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307 Uttar Pradesh, India.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Mar 03; Vol. 2021, pp. 8839479. Date of Electronic Publication: 2021 Mar 03 (Print Publication: 2021).
DOI: 10.1155/2021/8839479
Abstrakt: Black berry ( Syzygium cumini ) fruit is useful in curing diabetic complications; however, its role in diabetes-induced cardiomyopathy is not yet known. In this study, we investigated the regulation of gelatinase-B (MMP-9) by S. cumini methanol seed extract (MSE) in diabetic cardiomyopathy using real-time PCR, RT-PCR, immunocytochemistry, gel diffusion assay, and substrate zymography. The regulatory effects of MSE on NF- κ B, TNF- α , and IL-6 were also examined. Identification and estimation of polyphenol constituents present in S. cumini extract were carried out using reverse-phase HPLC. Further, in silico docking studies of identified polyphenols with gelatinase-B were performed to elucidate molecular level interaction in the active site of gelatinase-B. Docking studies showed strong interaction of S. cumini polyphenols with gelatinase-B. Our findings indicate that MSE significantly suppresses gelatinase-B expression and activity in high-glucose- (HG-) stimulated cardiomyopathy. Further, HG-induced activation of NF- κ B, TNF- α , and IL-6 was also remarkably reduced by MSE. Our results suggest that S. cumini MSE may be useful as an effective functional food and dietary supplement to regulate HG-induced cardiac stress through gelatinase.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2021 Neha Atale et al.)
Databáze: MEDLINE
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