Metagenomics of mine tailing rhizospheric communities and its selection for plant establishment towards bioremediation.

Autor: Romero MF; Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510, Coyoacán, Ciudad de México, Mexico; Posgrado en Ciencias Biológicas, Unidad de Posgrado, Universidad Nacional Autónoma de México, Ciudad de México, Mexico., Gallego D; Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510, Coyoacán, Ciudad de México, Mexico., Lechuga-Jiménez A; Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510, Coyoacán, Ciudad de México, Mexico; Departamento de Ecología Funcional, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico., Martínez JF; Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Hermosillo, Sonora, Mexico., Barajas HR; Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510, Coyoacán, Ciudad de México, Mexico., Hayano-Kanashiro C; Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Hermosillo, Mexico., Peimbert M; Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana, Unidad Cuajimalpa, Cuajimalpa, Ciudad de México, Mexico., Cruz-Ortega R; Departamento de Ecología Funcional, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico., Molina-Freaner FE; Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Hermosillo, Sonora, Mexico., Alcaraz LD; Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, 04510, Coyoacán, Ciudad de México, Mexico. Electronic address: lalcaraz@ciencias.unam.mx.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2021 Jun; Vol. 247, pp. 126732. Date of Electronic Publication: 2021 Mar 02.
DOI: 10.1016/j.micres.2021.126732
Abstrakt: Mining operations often generate tailing dams that contain toxic residues and are a source of contamination when left unconfined. The establishment of a plant community over the tailings has been proposed as a containment strategy known as phytostabilization. Previously, we described naturally occurring mine tailing colonizing plants such as Acacia farnesiana, Brickellia coulteri, Baccharis sarothroides, and Gnaphalium leucocephalum without finding local adaptation. We explored the rhizosphere microbes as contributors in plant establishment and described both the culturable and in situ diversity of rhizospheric bacteria using the 16S rRNA gene and metagenomic shotgun sequencing. We built a synthetic community (SC) of culturable rhizosphere bacteria from the mine tailings. The SC was then the foundation for a serial passes experiment grown in plant-derived nutrient sources, selecting for heavy metals tolerance, community cooperation, and competition. The outcome of the serial passes was named the 'final synthetic community' (FSC). Overall, diversity decreased from in situ uncultivable microbes from roots (399 bacteria genera) to the cultivated communities (291 genera), the SC (94 genera), and the lowest diversity was in the FSC (43 genera). Metagenomic diversity clustered into 94,245 protein families, where we found plant growth promotion-related genes such as the csgBAC and entCEBAH, coded in a metagenome-assembled genome named Kosakonia sp. Nacozari. Finally, we used the FSC to inoculate mine tailing colonizing plants in a greenhouse experiment. The plants with the FSC inocula observed higher relative plant growth rates in sterile substrates. The FSC presents promising features that might make it useful for phytostabilization tailored strategies.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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