AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets.

Autor:	Stadlbauer D; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Waal L; Viroclinics Biosciences B.V., Viroclinics Xplore, Schaijk, The Netherlands., Beaulieu E; GSK, Laval, QC, Canada.; Nexelis, Laval, QC, Canada., Strohmeier S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria., Kroeze EJBV; Viroclinics Biosciences B.V., Viroclinics Xplore, Schaijk, The Netherlands., Boutet P; GSK, Wavre, Belgium., Osterhaus ADME; Viroclinics Biosciences B.V., Viroclinics Xplore, Schaijk, The Netherlands.; The Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine, Hannover, Germany., Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Innis BL; GSK, King of Prussia, PA, USA.; PATH, Center for Vaccine Innovation and Access, Washington, DC, USA., Nachbagauer R; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Moderna Inc., Cambridge, MA, USA., Stittelaar KJ; Viroclinics Biosciences B.V., Viroclinics Xplore, Schaijk, The Netherlands.; Wageningen Bioveterinary Research, Wageningen University & Research, Lelystad, The Netherlands., Mallett CP; GSK, Laval, QC, Canada. corey.p.mallett@gsk.com.; GSK, Rockville, MD, USA. corey.p.mallett@gsk.com.
Jazyk:	angličtina
Zdroj:	NPJ vaccines [NPJ Vaccines] 2021 Mar 19; Vol. 6 (1), pp. 40. Date of Electronic Publication: 2021 Mar 19.
DOI:	10.1038/s41541-021-00299-3
Abstrakt:	Human infections with avian H7N9 subtype influenza viruses are a major public health concern and vaccines against H7N9 are urgently needed for pandemic preparedness. In early 2013, novel H7N9 influenza viruses emerged in China that caused about 1600 human cases of infection with a high associated case fatality rate. In this study, two H7N9 split virion vaccines with or without AS03 adjuvant were tested in the naive ferret model. Serological analyses demonstrated that homologous hemagglutination inhibition and microneutralization antibody titers were detectable in the ferrets after the first immunization with the AS03-adjuvanted vaccines that were further boosted by the second immunization. In addition, heterologous antibody titers against older H7 subtype viruses of the North American lineage (H7N7, H7N3) and newer H7 subtype viruses of the Eurasian lineage (H7N9) were detected in the animals receiving the AS03-adjuvanted vaccines. Animals receiving two immunizations of the AS03-adjuvanted vaccines were protected from weight loss and fever in the homologous challenge study and had no detectable virus in throat or lung samples. In addition, microscopic examination post-challenge showed animals immunized with the AS03-adjuvanted vaccines had the least signs of lung injury and inflammation, consistent with the greater relative efficacy of the adjuvanted vaccines. In conclusion, this study demonstrated that the AS03-adjuvanted H7N9 vaccines elicited high levels of homologous and heterologous antibodies and protected against H7N9 virus damage post-challenge.
Databáze:	MEDLINE
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