Statherin-derived peptides as antifungal strategy against Candida albicans.

Autor: Pellissari CVG; Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University, UNESP, 1680 Rua Humaitá, Araraquara, SP, 14801-385, Brazil; College of Dentistry, University of Saskatchewan, 105 Wiggins Road, Saskatoon, SK, S7N 5E4, Canada., Jorge JH; Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University, UNESP, 1680 Rua Humaitá, Araraquara, SP, 14801-385, Brazil. Electronic address: habib.jorge@unesp.br., Marin LM; College of Dentistry, University of Saskatchewan, 105 Wiggins Road, Saskatoon, SK, S7N 5E4, Canada., Sabino-Silva R; Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia, 3Q - 2121 Av. João Naves de Ávila, Uberlandia, MG, 38400-902, Brazil., Siqueira WL; College of Dentistry, University of Saskatchewan, 105 Wiggins Road, Saskatoon, SK, S7N 5E4, Canada.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2021 May; Vol. 125, pp. 105106. Date of Electronic Publication: 2021 Mar 16.
DOI: 10.1016/j.archoralbio.2021.105106
Abstrakt: Objective: The aim of this in vitro study was to evaluate the effect of statherin and its naturally occurring peptides (DR9-2, DR9, GE-12, IT-32, GQ-19, IP-18) on Candida albicans metabolism and biofilm development.
Design: After the killing assay, a peptide pellicle was formed on the bottom of a polystyrene plate at the IC 50 of each peptide. Over the peptide pellicle, Candida albicans biofilm (48 h) was grown. The peptides antimicrobial activity after the peptides treatment was evaluated by alamarBlue, total biofilm biomass and colony forming units (CFU) counting.
Results: The pellicle with statherin and the peptides (DR9-2, DR9, GE-12, IP-18, GQ-19) was able to reduce he viability of Candida albicans compared to the negative control. They also decreased cell proliferation by 20 % and total biomass. IT-32 showed the highest reduction in cell proliferation and biomass, which was similar to the positive control, histatin 5.
Conclusions: These results suggest that the naturally occuring peptides from statherin are able to decrease Candida albicans colonization and biofilm proliferation.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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