Facile Synthesis of a Croconaine-Based Nanoformulation for Optoacoustic Imaging and Photothermal Therapy.

Autor: Liu N; Chair of Biological Imaging, Center for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, 81675, Germany.; Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., O'Connor P; Institute of Medicinal Chemistry, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany.; Institute of Structural Biology, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Gujrati V; Chair of Biological Imaging, Center for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, 81675, Germany.; Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Gorpas D; Chair of Biological Imaging, Center for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, 81675, Germany.; Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Glasl S; Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Blutke A; Research Unit Analytical Pathology, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Walch A; Research Unit Analytical Pathology, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany., Kleigrewe K; Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich, Freising, 85354, Germany., Sattler M; Institute of Structural Biology, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany.; Bavarian NMR Center and Center for Integrated Protein Science Munich, Technical University of Munich, Garching, 85747, Germany., Plettenburg O; Institute of Medicinal Chemistry, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany.; Center for Biomolecular Research, Institute of Organic Chemistry, Leibniz Universität Hannover, Hannover, 30167, Germany., Ntziachristos V; Chair of Biological Imaging, Center for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, 81675, Germany.; Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg, 85764, Germany.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2021 May; Vol. 10 (9), pp. e2002115. Date of Electronic Publication: 2021 Mar 18.
DOI: 10.1002/adhm.202002115
Abstrakt: Near-infrared (NIR) light absorbing theranostic agents can integrate optoacoustic imaging and photothermal therapy for effective personalized precision medicine. However, most of these agents face the challenges of unstable optical properties, material-associated toxicity, and nonbiodegradability, all of which limit their biomedical application. Several croconaine-based organic agents able to overcome some of these limitations have been recently reported, but these suffer from complicated multistep synthesis protocols. Herein, the use of CR760, a croconaine dye with excellent optical properties, is reported for nanoparticle formulation and subsequent optoacoustic imaging and photothermal therapy. Importantly, CR760 can be conveniently prepared in a single step from commercially available materials. Furthermore, CR760 can be covalently attached, via a polyethylene glycol linker, to the α v β 3 integrin ligand c(RGDyC), resulting in self-assembled nanoparticles (NPs) with cancer-targeting capability. Such CR760RGD-NPs exhibit strong NIR absorption, high photostability, high optoacoustic generation efficiency, and active tumor-targeting, making them ideal candidates for optoacoustic imaging. Due to favorable electron transfer, CR760RGD-NPs display a 45.37% photothermal conversion efficiency thereby rendering them additionally useful for photothermal therapy. Targeted tumor elimination, biosafety, and biocompatibility are demonstrated in a 4T1 murine breast tumor model. This work points to the use of CR760RGD-NPs as a promising nanoagent for NIR-based cancer phototheranostics.
(© 2021 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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