Cytonemes with complex geometries and composition extend into invaginations of target cells.
| Autor: | Wood BM; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA., Baena V; Department of Cell Biology, University of Connecticut Health Center, Farmington, CT., Huang H; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA., Jorgens DM; Electron Microscope Laboratory, University of California, Berkeley, Berkeley, CA., Terasaki M; Department of Cell Biology, University of Connecticut Health Center, Farmington, CT., Kornberg TB; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA. |
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| Jazyk: | angličtina |
| Zdroj: | The Journal of cell biology [J Cell Biol] 2021 May 03; Vol. 220 (5). |
| DOI: | 10.1083/jcb.202101116 |
| Abstrakt: | Cytonemes are specialized filopodia that mediate paracrine signaling in Drosophila and other animals. Studies using fluorescence confocal microscopy (CM) established their general paths, cell targets, and essential roles in signaling. To investigate details unresolvable by CM, we used high-pressure freezing and EM to visualize cytoneme structures, paths, contents, and contacts. We observed cytonemes previously seen by CM in the Drosophila wing imaginal disc system, including disc, tracheal air sac primordium (ASP), and myoblast cytonemes, and identified cytonemes extending into invaginations of target cells, and cytonemes connecting ASP cells and connecting myoblasts. Diameters of cytoneme shafts vary between repeating wide (206 ± 51.8 nm) and thin (55.9 ± 16.2 nm) segments. Actin, ribosomes, and membranous compartments are present throughout; rough ER and mitochondria are in wider proximal sections. These results reveal novel structural features of filopodia and provide a basis for understanding cytoneme cell biology and function. (© 2021 Wood et al.) |
| Databáze: | MEDLINE |
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