Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis.

Autor: Cantiga-Silva C; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Estrela C; Department of Stomatologic Sciences, School of Dentistry, UFG - Federal University of Goiás, Goiânia, Brazil., Segura-Egea JJ; Endodontic Section, Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain., Azevedo JP; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., de Oliveira PHC; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Cardoso CBM; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Pinheiro TN; Department of Oral Pathology and Oral Medicine, Dental School of Amazonas State University, Manaus, Brazil., Ervolino E; Department of Basic Science, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Sivieri-Araújo G; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Cintra LTA; Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.; Dental Assistance Center for Disabled Persons (CAOE), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2021 Aug; Vol. 54 (8), pp. 1353-1361. Date of Electronic Publication: 2021 Apr 04.
DOI: 10.1111/iej.13519
Abstrakt: Aim: To investigate the effects of liver fibrosis (LF) on the pro-inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats.
Methodology: Forty male Wistar rats were distributed into four groups: C - control, AP - rats with AP, LF - rats with LF, AP + LF - rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α). Student's t-test and Mann-Whitney's U-test were used for statistical analysis (P < 0.05).
Results: Inflammatory infiltrate was moderate in the AP group and severe in the AP + LF group (P < 0.05). Periapical bone resorption was significantly larger in the AP + LF group compared with the AP group (P < 0.05). IL-1β, IL-6 and TNF-α levels were significantly higher in AP + LF group when compared to the AP group (P < 0.05).
Conclusion: More intense inflammatory infiltrate, greater amounts of pro-inflammatory cytokines and increased periapical bone resorption were observed in the presence of liver fibrosis in rats with exposed pulps.
(© 2021 International Endodontic Journal. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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