Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis.

Autor:	Teixeira Essenfelder L; Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Gomes AA; Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Coimbra JLM; Department of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Moreira MA; Department of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Ferraz SM; Department of Veterinary Medicine, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Miquelluti DJ; Department of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Felippe da Silva G; Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil., Magalhães MLB; Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2021 Mar 06; Vol. 26, pp. 100965. Date of Electronic Publication: 2021 Mar 06 (Print Publication: 2021).
DOI:	10.1016/j.bbrep.2021.100965
Abstrakt:	β-Glucosidases are enzymes present in all living organisms, playing a pivotal role in diverse biological processes. These enzymes cleave β-glycosidic bonds between carbohydrates, or between a carbohydrate and a non-carbohydrate moiety, which may result in the liberation of volatile aglycones. Released compounds execute diverse physiological roles , while the industry takes advantage of exogenously added β-glucosidases for aroma enrichment during food and beverage production. β-Glucosidase enzymatic activity has been reported in human saliva and given the fact that these enzymes are involved in aroma release, we investigated here the correlation between β-glucosidase activity in human saliva and the occurrence of halitosis. Measurement of salivary enzyme activity of 48 volunteers was performed using p -nitrophenyl-β-d-glucopyranoside as substrate. Each volunteer was clinically evaluated by a dental surgeon and clinical and laboratorial data were statistically analyzed. Gas-chromatography of saliva headspace allowed the analysis of the direct role of exogenous β-glucosidase on aromatic /volatile profile of saliva samples. The data demonstrated a positive correlation between halitosis and enzymatic activity, suggesting that the enzyme exerts a direct role in the occurrence of bad breath. Gas-chromatography analysis demonstrated that exogenously added enzyme led to the alteration of volatile organic content, confirming a direct contribution of β-glucosidase activity on saliva volatile compounds release. Although halitosis is a multifactorial condition, the complete understanding of all governing factors may allow the development of more effective treatment strategies. Such studies may pave the way to the use of β-glucosidase inhibitors for halitosis clinical management. Competing Interests: The authors declare no financial or commercial conflict of interest. (© 2021 The Authors.)
Databáze:	MEDLINE
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