Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.

Autor:	de Alcantara BN; Post-Graduate Programme in Virology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., Imbeloni AA; National Primate Centre, Evandro Chagas Institute, Highway BR-316, km 7, Ananindeua, Pará, 67030-000, Brazil., de Brito Simith Durans D; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., de Araújo MTF; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., do Rosário Moutinho da Cruz E; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., de Carvalho CAM; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil.; Pará State University, 2623 Perebebuí Lane, Belém, Pará, 66095-662, Brazil., de Mendonça MHR; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., de Sousa JR; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., Moraes AF; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., Filho AJM; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., de Lourdes Gomes Lima M; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., Neto OPA; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., Chiang JO; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil., de Azevedo Scalercio SRR; National Primate Centre, Evandro Chagas Institute, Highway BR-316, km 7, Ananindeua, Pará, 67030-000, Brazil., Carneiro LA; National Primate Centre, Evandro Chagas Institute, Highway BR-316, km 7, Ananindeua, Pará, 67030-000, Brazil., Quaresma JAS; Department of Pathology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil.; Pará State University, 2623 Perebebuí Lane, Belém, Pará, 66095-662, Brazil., da Costa Vasconcelos PF; Post-Graduate Programme in Virology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil.; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil.; Pará State University, 2623 Perebebuí Lane, Belém, Pará, 66095-662, Brazil., de Almeida Medeiros DB; Post-Graduate Programme in Virology, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil. danielemedeiros@iec.gov.br.; Department of Arbovirology and Haemorrhagic Fevers, Evandro Chagas Institute, BR-316 Highway, km 7, Ananindeua, Pará, 67030-000, Brazil. danielemedeiros@iec.gov.br.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2021 Mar 17; Vol. 11 (1), pp. 6099. Date of Electronic Publication: 2021 Mar 17.
DOI:	10.1038/s41598-021-85571-1
Abstrakt:	The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.
Databáze:	MEDLINE
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