| Autor: |
Thorsen J; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital.; Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, and., Stokholm J; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital.; Department of Food Science, Faculty of Science, and.; Department of Pediatrics, Slagelse Hospital, Slagelse, Denmark., Rasmussen MA; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital.; Department of Food Science, Faculty of Science, and., Mortensen MS; Section for Microbiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark; and., Brejnrod AD; Section for Microbiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark; and., Hjelmsø M; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital., Shah S; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital., Chawes B; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital., Bønnelykke K; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital., Sørensen SJ; Section for Microbiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark; and., Bisgaard H; Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital. |
| Abstrakt: |
Rationale: Childhood asthma is often preceded by recurrent episodes of asthma-like symptoms, which can be triggered by both viral and bacterial agents. Recent randomized controlled trials have shown that azithromycin treatment reduces episode duration and severity through yet undefined mechanisms. Objectives: To study the influence of the airway microbiota on the effect of azithromycin treatment during acute episodes of asthma-like symptoms. Methods: Children from the COPSAC 2010 (Copenhagen Prospective Studies on Asthma in Childhood 2010) cohort with recurrent asthma-like symptoms aged 12-36 months were randomized during acute episodes to azithromycin or placebo as previously reported. Before randomization, hypopharyngeal aspirates were collected and examined by 16S ribosomal RNA gene amplicon sequencing. Measurements and Main Results: In 139 airway samples from 68 children, episode duration after randomization was associated with microbiota richness (7.5% increased duration per 10 additional operational taxonomic units [OTUs]; 95% confidence interval, 1-14%; P = 0.025), with 15 individual OTUs (including several Neisseria and Veillonella ), and with microbial pneumotypes defined from weighted UniFrac distances (longest durations in a Neisseria -dominated pneumotype). Microbiota richness before treatment increased the effect of azithromycin by 10% per 10 additional OTUs, and more OTUs were positively versus negatively associated with an increased azithromycin effect (82 vs. 58; P = 0.0032). Furthermore, effect modification of azithromycin was found for five individual OTUs (three OTUs increased and two OTUs decreased the effect; q < 0.05). Conclusions: The airway microbiota in acute episodes of asthma-like symptoms is associated with episode duration and modifies the effect of azithromycin treatment of the episodes in preschool children with recurrent asthma-like symptoms. Clinical trial registered with www.clinicaltrials.gov (NCT01233297). |
