| Autor: |
Sheen W; Neonatal-Perinatal Medicine, Cohen Children's Medical Center, New Hyde Park, New York, USA.; Lilling Family Neonatal Research Lab, Feinstein Institutes for Medical Research, Manhasset, New York, USA.; Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA., Ahmed M; Neonatal-Perinatal Medicine, Cohen Children's Medical Center, New Hyde Park, New York, USA.; Lilling Family Neonatal Research Lab, Feinstein Institutes for Medical Research, Manhasset, New York, USA.; Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA., Patel H; Neonatal-Perinatal Medicine, Cohen Children's Medical Center, New Hyde Park, New York, USA.; Lilling Family Neonatal Research Lab, Feinstein Institutes for Medical Research, Manhasset, New York, USA.; Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA., Codipilly CN; Neonatal-Perinatal Medicine, Cohen Children's Medical Center, New Hyde Park, New York, USA.; Lilling Family Neonatal Research Lab, Feinstein Institutes for Medical Research, Manhasset, New York, USA.; Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA., Schanler RJ; Neonatal-Perinatal Medicine, Cohen Children's Medical Center, New Hyde Park, New York, USA.; Lilling Family Neonatal Research Lab, Feinstein Institutes for Medical Research, Manhasset, New York, USA.; Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA. |
| Abstrakt: |
Background: In the neonatal intensive care unit (NICU) expressed mothers' milk usually is stored frozen until used. We found that when human milk was stored at -20°C for up to 9 months there were reduced bacterial counts and pH, increased free fatty acids, but unchanged immune proteins. Antioxidant protection is an important benefit of human milk. Few studies have evaluated long-term effects of cold storage on the antioxidant capacity of human milk. We hypothesized that the antioxidant capacity of human milk is affected adversely by long-term storage at -20°C. Objective: To study the impact of long-term cold storage on the oxidative capacity of human milk and the biological impact of these changes on macromolecular constituents of human milk. Methods: Freshly expressed milk was obtained from mothers in the NICU, stored at -20°C for 6 months, and compared with the baseline. Paired samples were analyzed for glutathione, hydrogen peroxide (H 2 O 2 ), 8-isoprostane, catalase, and superoxide dismutase. Results: There was no change in H 2 O 2 concentration between baseline and 6 months. Significant reductions from baseline in both catalase and superoxide dismutase concentrations and activities, total glutathione, oxidized glutathione, reduced glutathione, and the ratio of reduced to oxidized glutathione were observed ( p < 0.05). There was a significant increase in 8-isoprostane concentrations ( p < 0.001). Conclusion: These data indicate significant changes in antioxidant capacity of human milk, including oxidation of macromolecules, after storage at -20°C for 6 months. The clinical implication of these findings may explain the nonuniform protection against oxidant disease in preterm infants fed human milk. |
