The Effect of LPS and Flagellin on the Process of Lipolysis in Mesenchymal Stromal Cells during Adipogenic Differentiation.

Autor: Mishra A; Dmitry Rogachev State National Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of health of the Russian Federation, Moscow, Russia. mishra@phystech.edu.; Moscow Institute of Physics and Technology (MIPT), Dolgoprudny, Moscow region, Russia. mishra@phystech.edu., Tsypandina EV; V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia., Gaponov AM; Dmitry Rogachev State National Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of health of the Russian Federation, Moscow, Russia., Rumyantsev SA; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Shestopalov AV; Dmitry Rogachev State National Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Feb; Vol. 170 (4), pp. 571-574. Date of Electronic Publication: 2021 Mar 16.
DOI: 10.1007/s10517-021-05108-4
Abstrakt: We analyzed the effects of bacterial pathogen-associated molecular patterns (LPS and flagellin) and adrenergic agonist isoproterenol on the content of total and phosphorylated (Ser552) hormone-sensitive lipase in mesenchymal stromal cells and cell products of their adipogenic differentiation. The expression of hormone-sensitive lipase and an increase in the content of its activated phosphorylated form were demonstrated by Western blotting in cells of all three lines of adipogenic differentiation. Under the influence of flagellin, the content of total and phosphorylated forms of hormone-sensitive lipase increased in brown adipocytes, while LPS induced a decrease in the content of total hormone-sensitive lipase in white adipocytes. We hypothesize that bacterial pathogen-associated molecular patterns can activate lipolysis under pathological conditions associated with slow remodeling of the adipose tissue.
Databáze: MEDLINE
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