Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress.

Autor: Chuong NN; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Hoang XLT; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Nghia DHT; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Nguyen NC; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Thao DTT; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Tran TB; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Ngoc TTM; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Thu NBA; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Nguyen QT; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam., Thao NP; Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam. npthao@hcmiu.edu.vn.; Vietnam National University, Linh Trung Ward, Thu Duc, Ho Chi Minh, 700000, Vietnam. npthao@hcmiu.edu.vn.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2021 May; Vol. 40 (5), pp. 819-834. Date of Electronic Publication: 2021 Mar 16.
DOI: 10.1007/s00299-021-02677-6
Abstrakt: Key Message: Ectopic expression of Glycine max two-component system member GmHP08 in Arabidopsis enhanced drought tolerance of transgenic plants, possibly via ABA-dependent pathways. Phosphorelay by two-component system (TCS) is a signal transduction mechanism which has been evolutionarily conserved in both prokaryotic and eukaryotic organisms. Previous studies have provided lines of evidence on the involvement of TCS genes in plant perception and responses to environmental stimuli. In this research, drought-associated functions of GmHP08, a TCS member from soybean (Glycine max L.), were investigated via its ectopic expression in Arabidopsis system. Results from the drought survival assay showed that GmHP08-transgenic plants exhibited higher survival rates compared with their wild-type (WT) counterparts, indicating better drought resistance of the former group. Analyses revealed that the transgenic plants outperformed the WT in various regards, i.e. capability of water retention, prevention of hydrogen peroxide accumulation and enhancement of antioxidant enzymatic activities under water-deficit conditions. Additionally, the expression of stress-marker genes, especially antioxidant enzyme-encoding genes, in the transgenic plants were found greater than that of the WT plants. In contrary, the expression of SAG13 gene, one of the senescence-associated genes, and of several abscisic acid (ABA)-related genes was repressed. Data from this study also revealed that the ectopic expression lines at germination and early seedling development stages were hypersensitive to exogenous ABA treatment. Taken together, our results demonstrated that GmHP08 could play an important role in mediating plant response to drought, possibly via an ABA-dependent manner.
Databáze: MEDLINE
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