Engineering motile aqueous phase-separated droplets via liposome stabilisation.

Autor: Zhang S; Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK., Contini C; Department of Chemical Engineering, Exhibition Road, Imperial College London, London, SW7 2AZ, UK.; fabriCELL, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK., Hindley JW; Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK.; fabriCELL, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK.; Institute of Chemical Biology, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK., Bolognesi G; Department of Chemical Engineering, Loughborough University, Loughborough, LE11 3TU, UK., Elani Y; Department of Chemical Engineering, Exhibition Road, Imperial College London, London, SW7 2AZ, UK.; fabriCELL, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK.; Institute of Chemical Biology, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK., Ces O; Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK. o.ces@imperial.ac.uk.; fabriCELL, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK. o.ces@imperial.ac.uk.; Institute of Chemical Biology, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London, W12 0BZ, UK. o.ces@imperial.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Mar 15; Vol. 12 (1), pp. 1673. Date of Electronic Publication: 2021 Mar 15.
DOI: 10.1038/s41467-021-21832-x
Abstrakt: There are increasing efforts to engineer functional compartments that mimic cellular behaviours from the bottom-up. One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems. Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems. However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases. Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration. We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient. The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.
Databáze: MEDLINE
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