In Vitro Activation and Development of Goat Preantral Follicles Enclosed in Ovarian Tissue Co-cultured with Mesenchymal Stem Cells.

Autor: Sousa RP; Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil., Duarte ABG; Department of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil., Pinto Y; Laboratory of Manipulation of Oocytes enclosed in Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Sá NAR; Laboratory of Manipulation of Oocytes enclosed in Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Alves BG; Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Cibin FWS; Breeding Biotechnology Laboratory (Biotech), Federal University of Pampa, Uruguaiana, Brazil., Silva GC; Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil., Carvalho CES; Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil., Argôlo Neto NM; Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes enclosed in Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Silva CMG; Humid Tropics Research Institute, Federal University of Southern and Southeastern Pará, Xinguara, Brazil., Figueiredo JR; Laboratory of Manipulation of Oocytes enclosed in Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Carvalho MAM; Integrated Nucleus of Morphology and Research with Stem Cells (NUPCelt), Federal University of Piauí, Teresina, PI, Brazil. mcelina@ufpi.edu.br.; Department of Veterinary Morphophysiology, Federal University of Piauí, Rua Dirce de Oliveira, 3397, Bairro Ininga, Teresina, Piauí, Brazil. mcelina@ufpi.edu.br.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Jun; Vol. 28 (6), pp. 1709-1717. Date of Electronic Publication: 2021 Mar 15.
DOI: 10.1007/s43032-021-00540-3
Abstrakt: The development of culture systems capable of maintaining follicular growth since the preantral stage has been the target of investigations. Mesenchymal stem cells (MSC) present potential for use in a wide range of applications, including research aimed at preserving fertility. Therefore, this study investigated the use of caprine Wharton's Jelly Mesenchymal Stem Cells (WJMSC) on the survival and in vitro development of goat preantral follicles enclosed in ovarian fragments cultured for 1 or 7 days. Fragments of the ovarian cortex were immediately fixed (non-cultured control) or distributed in four treatments: ovarian tissue cultured in control medium (α-MEM + ); ovarian tissue cultured in α-MEM + supplemented with FBS (α-MEM + + FBS); ovarian tissue co-cultured with stem cells in α-MEM + (α-MEM + + SC); and ovarian tissue co-cultured with stem cell in α-MEM + + FBS (α-MEM + + SC + FBS). The rates of cell proliferation, follicular survival, and activation, as well as follicular diameter, were evaluated. After 7 days, the treatment co-cultured with stem cells showed a higher (P < 0.05) percentage of morphologically normal preantral follicles compared to the other treatments, as well as a higher (P < 0.05) activation rate compared to cultured control. Moreover, the follicular diameter was higher (P < 0.05) in the treatment co-cultured with stem cells compared to co-cultured with stem cells plus FBS. This study demonstrates for the first time that in vitro co-culture of caprine WJMSC with preantral follicles enclosed in goat ovarian tissue improves activation and early follicular development.
Databáze: MEDLINE
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