Roundabout homolog 1 inhibits proliferation via the YY1-ROBO1-CCNA2-CDK2 axis in human pancreatic cancer.

Autor: Chen Q; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Shen P; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Ge WL; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Yang TY; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Wang WJ; Nanjing University of Chinese Medicine, Nanjing, China., Meng LD; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Huang XM; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Zhang YH; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Cao SJ; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Miao Y; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; Pancreas Institute, Nanjing Medical University, Nanjing, China., Jiang KR; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China. jiangkuirong@njmu.edu.cn.; Pancreas Institute, Nanjing Medical University, Nanjing, China. jiangkuirong@njmu.edu.cn., Zhang JJ; Pancreas Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China. zhangjingjing@njmu.edu.cn.; Pancreas Institute, Nanjing Medical University, Nanjing, China. zhangjingjing@njmu.edu.cn.
Jazyk: angličtina
Zdroj: Oncogene [Oncogene] 2021 Apr; Vol. 40 (15), pp. 2772-2784. Date of Electronic Publication: 2021 Mar 13.
DOI: 10.1038/s41388-021-01741-5
Abstrakt: Pancreatic cancer (PC) is highly malignant and has a high mortality with a 5-year survival rate of less than 8%. As a member of the roundabout immunoglobulin superfamily of proteins, ROBO1 plays an important role in embryogenesis and organogenesis and also inhibits metastasis in PC. Our study was designed to explore whether ROBO1 has effects on the proliferation of PC and its specific mechanism. The expression of ROBO1 was higher in cancer tissues than in matched adjacent tissues by immunohistochemistry (IHC) and qRT-PCR. Low ROBO1 expression is associated with PC progression and poor prognosis. Overexpression of ROBO1 can inhibit the proliferation of PC cells in vitro, and the S phase fraction can also be induced. Further subcutaneous tumor formation in nude mice showed that ROBO1 overexpression can significantly inhibit tumor growth. YY1 was found to directly bind to the promoter region of ROBO1 to promote transcription by a luciferase reporter gene assay, a chromatin immunoprecipitation (ChIP) and an electrophoretic mobility shift assay (EMSA). Mechanistic studies showed that YY1 can inhibit the development of PC by directly regulating ROBO1 via the CCNA2/CDK2 axis. Taken together, our results suggest that ROBO1 may be involved in the development and progression of PC by regulating cell proliferation and shows that ROBO1 may be a novel and promising therapeutic target for PC.
Databáze: MEDLINE
Externí odkaz: