Use of magnetic Fe 3 O 4 nanoparticles coated with bovine serum albumin for the separation of lysozyme from chicken egg white.

Autor: Santos MG; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil. Electronic address: mariane.goncalves@unifal-mg.edu.br., de Carvalho DT; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Caminitti LB; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., de Lima BBA; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Cavalcanti MHDS; Colloid Chemistry Group, Chemistry Institute, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Dos Santos DFR; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Virtuoso LS; Colloid Chemistry Group, Chemistry Institute, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Hirata DB; Bioprocess Laboratory - BIOPRO, Chemistry Institute, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil., Figueiredo EC; Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000 Alfenas, MG, Brazil.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2021 Aug 15; Vol. 353, pp. 129442. Date of Electronic Publication: 2021 Mar 03.
DOI: 10.1016/j.foodchem.2021.129442
Abstrakt: Fe 3 O 4 magnetic nanoparticles modified with tetraethyl orthosilicate and bovine serum albumin (Fe 3 O 4 @TEOS@BSA) were synthesized and efficiently used to separate lysozyme from egg white. Glutaraldehyde was used to crosslink the bovine serum albumine molecules around the nanoparticles. The surface modifications were attested by transmission electron microscopy, infrared spectroscopy, thermogravimetry analysis, and zeta potential. The material was thermally stable, and its surface charge was pH-dependent. The best lysozyme adsorption and desorption were obtained at pHs 10.0 and 5.0, respectively. The pseudo-second-order model fitted well into the lysozyme adsorption kinetic data and the time for the equilibrium was 15 min. The adsorption equilibrium results were best described by the Freundlich model. Fe 3 O 4 @TEOS@BSA particles were very efficient to extract lysozyme from chicken egg, according to the SDS-PAGE analyses. The extracted molecules maintained their enzymatic activity in about 90%. Fe 3 O 4 @TEOS@BSA particles were easily recycled, with their reuse being possible 5 times with the same performance.
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Databáze: MEDLINE
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