Prototype of a DNA Vaccine against Marburg Virus.

Autor: Volkova NV; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. tasha_wolkowa11.93@mail.ru., Pyankov OV; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Ivanova AV; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Isaeva AA; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Zybkina AV; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Kazachinskaya EI; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia., Shcherbakov DN; State Research Centre of Virology and Biotechnology 'Vector', Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Feb; Vol. 170 (4), pp. 475-478. Date of Electronic Publication: 2021 Mar 13.
DOI: 10.1007/s10517-021-05091-w
Abstrakt: The preparation and study of the biological properties of the pVAKS-GPVM DNA immunogen containing a gene encoding Marburgvirus glycoprotein are described. The specificity of blood serum antibodies of guinea pigs immunized with DNA immunogen was analyzed by ELISA. Inactivated viral preparation, recombinant glycoprotein (GP) obtained in the prokaryotic system and virus-like particles based on the recombinant vesicular stomatitis virus exhibiting Marburgvirus GP were used as the antigens. The neutralizing activity of antibodies of immunized animals was tested in vitro using a pseudovirus system. It was demonstrated that the developed immunogen administered to guinea pigs induced the production of specific antibodies that neutralize virus-like particles and Marburgvirus in cultured Vero cells.
Databáze: MEDLINE
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