Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with 99m Tc for Differential Diagnosis of Bone Infection of the Limbs.

Autor: Zavadovskaya VD; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Larkina MS; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.; National Research Tomsk Polytechnic University, Tomsk, Russia., Stasyuk ES; National Research Tomsk Polytechnic University, Tomsk, Russia., Zorkaltsev MA; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Udodov VD; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Ivanov VV; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Shevtsova NM; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Rogov AS; National Research Tomsk Polytechnic University, Tomsk, Russia., Lushchyk AY; Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus., Yantsevich AV; Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus., Belousov MV; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.; National Research Tomsk Polytechnic University, Tomsk, Russia., Baikov AN; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia., Skuridin VS; National Research Tomsk Polytechnic University, Tomsk, Russia., Pershina AG; Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia. allysyz@mail.ru.; National Research Tomsk Polytechnic University, Tomsk, Russia. allysyz@mail.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Feb; Vol. 170 (4), pp. 415-419. Date of Electronic Publication: 2021 Mar 13.
DOI: 10.1007/s10517-021-05078-7
Abstrakt: We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99m Тс-DPAH-UBI29-41 and 99m Тс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.
Databáze: MEDLINE
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