DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis.

Autor: Cañibano E; Centro Nacional de Biotecnología, CNB-CSIC, Madrid 28049, Spain., Bourbousse C; Institut de biologie de l'École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris 75005, France., García-León M; Centro Nacional de Biotecnología, CNB-CSIC, Madrid 28049, Spain., Garnelo Gómez B; Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China., Wolff L; Institut de biologie de l'École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris 75005, France., García-Baudino C; Centro Nacional de Biotecnología, CNB-CSIC, Madrid 28049, Spain., Lozano-Durán R; Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, 72076 Tübingen, Germany., Barneche F; Institut de biologie de l'École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, Université PSL, Paris 75005, France., Rubio V; Centro Nacional de Biotecnología, CNB-CSIC, Madrid 28049, Spain. Electronic address: vrubio@cnb.csic.es., Fonseca S; Centro Nacional de Biotecnología, CNB-CSIC, Madrid 28049, Spain. Electronic address: sfonseca@cnb.csic.es.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2021 Jun 07; Vol. 14 (6), pp. 963-982. Date of Electronic Publication: 2021 Mar 09.
DOI: 10.1016/j.molp.2021.03.009
Abstrakt: DE-ETIOLATED 1 (DET1) and CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) are two essential repressors of Arabidopsis photomorphogenesis. These proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligases that mediate the degradation of several photomorphogenic transcription factors, including ELONGATED HYPOCOTYL 5 (HY5), thereby controlling multiple gene-regulatory networks. Despite extensive biochemical and genetic analyses of their multi-subunit complexes, the functional links between DET1 and COP1 have long remained elusive. Here, we report that DET1 associates with COP1 in vivo, enhances COP1-HY5 interaction, and promotes COP1 destabilization in a process that dampens HY5 protein abundance. By regulating its accumulation, DET1 avoids HY5 association with hundreds of second-site genomic loci, which are also frequently targeted by the skotomorphogenic transcription factor PHYTOCHROME-INTERACTING FACTOR 3. Accordingly, ectopic HY5 chromatin enrichment favors local gene repression and can trigger fusca-like phenotypes. This study therefore shows that DET1-mediated regulation of COP1 stability tunes down the HY5 cistrome, avoiding hyper-photomorphogenic responses that might compromise plant viability.
(Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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