Quantitative analysis of differential dehydrin regulation in pine and spruce seedlings under water deficit.

Autor: Kartashov AV; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia. Electronic address: botanius@yandex.ru., Zlobin IE; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia., Pashkovskiy PP; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia., Pojidaeva ES; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia., Ivanov YV; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia., Mamaeva AS; Laboratory of Functional Genomics and Plant Proteomics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation., Fesenko IA; Laboratory of Functional Genomics and Plant Proteomics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation., Kuznetsov VV; K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., Moscow, 127276, Russia.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2021 May; Vol. 162, pp. 237-246. Date of Electronic Publication: 2021 Mar 03.
DOI: 10.1016/j.plaphy.2021.02.040
Abstrakt: Dehydrins are well-known components of plant responses to different stresses that cause dehydration, including drought, freezing, salinity, etc. In conifers, the dehydrin gene family is very large, implying that the members of this family have important physiological functions in conifer stress tolerance. However, dehydrin gene expression displays a wide range of responses to stress, from thousand-fold increased expression to decreased expression, and it is generally unknown how regulatory systems are connected at the mRNA and protein levels. Therefore, we studied these aspects of dehydrin regulation in Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) H. Karst) seedlings under polyethylene glycol 6000-induced osmotic stress ranging from relatively low (culture medium water potential of -0.15 MPa) to very high (-1.0 MPa) intensities. In pine, the major dehydrin protein was Dhn1 in both the roots and needles, and in spruce, two isoforms of the Dhn4 protein were the major dehydrins; both of these proteins are AESK-type dehydrins. The genes encoding these major proteins were highly expressed even under control conditions; surprisingly, we also observed several highly expressed dehydrin genes that were not abundantly translated. Under osmotic stress, the most prominent expression changes were observed for the dehydrin genes with low basal expression levels, whereas highly expressed genes generally demonstrated rather modest changes in expression. We report proposed constitutive physiological functions of the AESK-type dehydrins in Pinaceae plants.
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Databáze: MEDLINE
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