Photoprotective activity of zirconia nanoparticles.

Autor: de Sá RG; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil., Arantes TM; Department of Chemistry, Federal University of Jataí, GO, Brazil., de Macedo EF; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil., Dona' LM; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil., Pereira JCF; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil., Hurtado CR; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil., Varghese RJ; Department of Chemical Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa., Oluwafemi OS; Department of Chemical Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa., Tada DB; Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo, SP, Brazil. Electronic address: d.tada@unifesp.br.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2021 Jun; Vol. 202, pp. 111636. Date of Electronic Publication: 2021 Feb 18.
DOI: 10.1016/j.colsurfb.2021.111636
Abstrakt: The increasing incidence of diseases caused by the harmful effects of UV radiation in skin, predominantly skin cancer, induce the search for more efficient photoprotector agents. Nowadays, titanium dioxide (TiO 2 ) and zinc oxide (ZnO) are the most widely used photoprotectors and therefore form the main components of commercially available sunscreens. Although the outstanding efficiency in absorbing and scattering UV radiation, mainly as nanoparticles, recent studies have raised concerns regarding the safe use of these nanoparticles, especially due to their high generation of reactive oxygen species (ROS). Thereby, this work focus on the evaluation of the photoprotective activity of zirconia nanoparticles (ZrO 2 NPs) and their cytotoxicity study in the presence and absence of UV irradiation. The ZrO 2 NPs were synthesized by hydrothermal method and their hydrodynamic diameter, Zeta potential and colloidal stability were characterized by dynamic light scattering. The morphology and size were observed by transmission electron microscopy. The synthesis resulted in ZrO 2 NPs with 50 nm of diameter and 56 nm of hydrodynamic diameter. The high colloidal stability was evidenced by the high value of Zeta potential (+48 mV) and low polydispersity index (0.09). The UV-vis spectrum of the ZrO 2 NPs in aqueous suspension showed an intense light scattering below 250 and a wide absorption band at 285 nm. The poor generation of ROS by ZrO 2 NPs was confirmed by the absence of photodegradation of methylene blue after long periods of irradiation. The in vitro assays performed with HaCaT cell line showed that the cell viability did not decrease in the absence of irradiation. However, after 24 h of incubation, the cell viability decreased under UV-irradiation in comparison with irradiated cells that were not incubated with ZrO 2 NPs. Notably, in these assays, the cells were incubated with the ZrO 2 NPs and after 24 h, they were replaced by fresh culture medium before the cell viability assay. Nevertheless, another in vitro assay was performed in order to evaluate the photoprotective activity of ZrO 2 NPs. The cells were irradiated in the presence of ZrO 2 NPs suspension. In this case, cell viability did not decrease even after long period of UV-irradiation and at higher concentration of ZrO 2 NPs. The present results showed that ZrO 2 NPs could be an interesting material to be used for skin photoprotection since they showed low cytotoxicity, absence of ROS generation and protection under UV irradiation. Additionally, the ZrO 2 NPs suspension was transparent as usually required for applications in sunscreens.
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Databáze: MEDLINE
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