| Autor: |
D'Imprima E; Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Max von Laue Strasse 3, 60438, Germany., Kühlbrandt W; Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Max von Laue Strasse 3, 60438, Germany. |
| Jazyk: |
angličtina |
| Zdroj: |
Quarterly reviews of biophysics [Q Rev Biophys] 2021 Mar 11; Vol. 54, pp. e4. Date of Electronic Publication: 2021 Mar 11. |
| DOI: |
10.1017/S0033583521000020 |
| Abstrakt: |
CryoEM has become the method of choice for determining the structure of large macromolecular complexes in multiple conformations, at resolutions where unambiguous atomic models can be built. Two effects that have limited progress in single-particle cryoEM are (i) beam-induced movement during image acquisition and (ii) protein adsorption and denaturation at the air-water interface during specimen preparation. While beam-induced movement now appears to have been resolved by all-gold specimen support grids with very small holes, surface effects at the air-water interface are a persistent problem. Strategies to overcome these effects include the use of alternative support films and new techniques for specimen deposition. We examine the future potential of recording perfect images of biological samples for routine structure determination at atomic resolution. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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