Anti-Trypanosoma cruzi activity, cytotoxicity, and chemical characterization of extracts from seeds of Lonchocarpus cultratus.

Autor:	Griebler A; Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Weyand Banhuk F; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Staffen IV; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Antunes Maciel Bortoluzzi A; Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Soprani Ayala T; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Ferreira Gandra R; Laboratory of Microbiology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Schuquel ITA; Department of Chemistry, Center of Exact Sciences, State University of Maringá, Maringá, PR, Brazil., Alves da Silva EA; Center of Exact and Technological Sciences, Western Parana State University, Cascavel, PR, Brazil., Marinho Jorge TC; Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil., Andrade Menolli R; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil. rafael.menolli@unioeste.br.
Jazyk:	angličtina
Zdroj:	Journal of infection in developing countries [J Infect Dev Ctries] 2021 Mar 07; Vol. 15 (2), pp. 270-279. Date of Electronic Publication: 2021 Mar 07.
DOI:	10.3855/jidc.12669
Abstrakt:	Introduction: Trypanosoma cruzi is the agent of Chagas' disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile. Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells. Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells. Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2021 Aline Griebler, Fernanda Weyand Banhuk, Izabela Virginia Staffen, Aline Antunes Maciel Bortoluzzi, Thais Soprani Ayala, Rinaldo Ferreira Gandra, Ivania Teresinha Albrecht Schuquel, Edson Antonio Alves da Silva, Tereza Cristina Marinho Jorge, Rafael Andrade Menolli.)
Databáze:	MEDLINE
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