Novel chimeric E2CD154 subunit vaccine is safe and confers long lasting protection against classical swine fever virus.

Autor: Suárez-Pedroso M; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba. Electronic address: marisela.suarez@cigb.edu.cu., Sordo-Puga Y; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Sosa-Teste I; Centro para la Producción de Animales de Laboratorio (CENPALAB), Centro de Toxicología Experimental (CETEX), Cuba., Rodriguez-Molto MP; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Naranjo-Valdés P; Laboratorio de Control Estatal, Cuba., Sardina-González T; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Santana-Rodríguez E; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Montero-Espinosa C; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Frías-Laporeaux MT; Centro Nacional de Sanidad Agropecuaria (CENSA), Apdo 10, San José de Las Lajas, Havana, Cuba., Fuentes-Rodríguez Y; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Pérez-Pérez D; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Oliva-Cárdenas A; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Pereda CL; Centro Nacional de Sanidad Agropecuaria (CENSA), Apdo 10, San José de Las Lajas, Havana, Cuba., González-Fernández N; Departamento de Desarrollo de Procesos, Centro de Ingeniería Genética y Biotecnología (CIGB), Camagüey, Cuba., Bover-Fuentes E; Departamento de Desarrollo de Procesos, Centro de Ingeniería Genética y Biotecnología (CIGB), Camagüey, Cuba., Vargas-Hernández M; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Duarte CA; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba., Estrada-García MP; Departamento de Biotecnología Animal, Centro de Ingeniería Genética y Biotecnología (CIGB), Havana, Cuba.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2021 Apr; Vol. 234, pp. 110222. Date of Electronic Publication: 2021 Mar 05.
DOI: 10.1016/j.vetimm.2021.110222
Abstrakt: E2CD154 is a vaccine candidate against classical swine fever (CSF) based on a chimeric protein composed of the E2 glycoprotein fused to porcine CD154 antigen, and formulated in the oil adjuvant Montanide™ ISA 50 V2. This vaccine confers early protection in pigs and prevents vertical transmission in pregnant sows. The objectives of this study were to assess the safety of this immunogen in piglets, to compare several doses of antigen in the formulation, and to study the duration of the immunity provided by this vaccine for up to 9 months. Three trials were conducted by immunizing pigs with a two-dose regime of the vaccine. Challenge experiments were carried out with the highly pathogenic Margarita strain. No local or systemic adverse effects were documented, and neither macroscopic nor microscopic pathological findings were observed in the vaccinated animals. The three antigen doses explored were safe and induced CSF protective neutralizing antibodies. The dose of 50 μg was selected for further development because it provided the best clinical and virological protection. Finally, this protective immunity was sustained for at least 9 months. This study demonstrates that E2CD154 vaccine is safe; defines a vaccine dose of 50 μg antigen, and evidences the capacity of this vaccine to confer long term protection from CSFV infection for up to 9 months post- vaccination. These findings complement previous data on the evaluation of this vaccine candidate, and suggest that E2CD154 is a promising alternative to modified live vaccines in CSF endemic areas.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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