Immunological screening of Lassa Virus among Health workers and Contacts of patients of Lassa fever in Ondo State.

Autor: Shaibu JO; Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria. Electronic address: josephshaibu2013@gmail.com., Salu OB; Centre for Human and Zoonotic Virology, Central Research Laboratory and the Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, PM.B. 12003, Idi-Araba, Surulere, Lagos, Nigeria., Amoo OS; Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria., Idigbe I; Clinical Sciences Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria., Musa AZ; Clinical Sciences Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria., Ezechi OC; Clinical Sciences Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria., Abejegah C; Infection Control and Research Centre, Federal Medical Centre, Owo, Ondo State, Nigeria., Ayodeji O; Infection Control and Research Centre, Federal Medical Centre, Owo, Ondo State, Nigeria., Salako BL; Clinical Sciences Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria., Omilabu SA; Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria; Centre for Human and Zoonotic Virology, Central Research Laboratory and the Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, PM.B. 12003, Idi-Araba, Surulere, Lagos, Nigeria., Audu RA; Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, 6 Edmond Crescent, P.M.B 2013, Yaba 101012, Lagos, Nigeria.
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2021 May; Vol. 226 (3), pp. 152076. Date of Electronic Publication: 2021 Mar 02.
DOI: 10.1016/j.imbio.2021.152076
Abstrakt: Background: The increasing trends of morbidity and mortality of Lassa fever is becoming more alarming in Nigeria. Information about immune response to the virus is limited. At exposure, the level of immunity plays a vital role in the vulnerability of individuals infected.
Objective: Investigating the immune status of health workers, infected cases and contacts of infected cases of Lassa fever in Ondo State.
Study Design: Blood samples were collected from 233 individuals comprising 102 health workers, 22 infected cases and 109 contacts of infected cases from Owo and Ose Local Government Areas and transported in triple level packaging. Plasma samples were analyzed for IgG and IgM markers using ReLASV® Pan-Lassa NP IgG/IgM ELISA Kit (Zalgen Labs, LLC, USA) while RNAs extracted from IgM positive samples were analyzed for LASV RNA according to manufacturers' instructions.
Result: Among the health workers, 20/102 (19.6%) and 2/102 (2.0%) were IgG and IgM positive respectively. While 16/22 (72.7%) and 14/22 (63.6%) were IgG and IgM positive respectively among the infected cases. Of the contacts of infected cases screened, 64/109 (58.7%) were IgG positive while 4/109 (3.7%) were positive for IgM. There was no detectable LASV RNA in the samples analyzed.
Conclusion: These findings suggest that majority of the health workers are naïve to the virus and hence may be prone to the viral infection. It could also be suggestive that a good personal protective procedure is been practiced by the health workers, hence the low exposure. However, most of the contacts of infected cases show exposure to the virus.
(Copyright © 2021. Published by Elsevier GmbH.)
Databáze: MEDLINE
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